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Dration buffer (pI 4?: 7 M Urea, 2 M Thiourea, 4 Chaps, 5 Isopropanol, 2.5 Glycerol, 1 DTT, 5 /ml Bio-Lytes 3/10 (Bio-Rad), inhibitor mix) or in 30 lysis buffer (pI 6?: 7 M Urea, 2 M Thiourea, 4 Chaps, 5 /ml IPG buffer pH 6?1 (Amersham Biosciences), 10 mM DTT, inhibitor mix). Samples were incubated with shaking for 15 min at 8 after the addition of 0.5 Benzonase (25 U/ > 99 purity, N

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