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Gested with trypsin as described previously [16]: after washing with water, water was removed and gel pieces were shrunk by dehydration with 50 acetonitrile for 15 min. Acetonitrile was removed and the proteins were reduced with 50 10 mM dithiothreitol in 100 mM NH4HCO3 for 30 min at 56 . The solution was removed, gel pieces were dehydrated as before and alkylated with 55 mM iodoacetamide in
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Ki M, Kitagaki H, Yamaji S, Sakamoto S, Matsuda K, Mori E: Reduction of cerebellar glucose metabolism in advanced Alzheimer's disease. J Nucl Med 1997, 38(6):925-928. 58. Larner AJ: The cerebellum in Alzheimer's disease. Dement Geriatr Cogn Disord 1997, 8(4):203-209. 59. Wegiel J, Wisniewski HM, Dziewiatkowski J, Badmajew E, Tarnawski M, Reisberg B, Mlodzik B, De Leon MJ, Miller DC: Cerebellar at
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Strategy to have the arrest data very fast. Learn how to do it through an online reports collection
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